| Advice on Conducting a Targeted Pathogenic Species Search using ITS1 data using QIIME2 feature-classifier plugin | | 3 | 53 | Thu Sep 24 2026 |
| 16S Sequencing for Species-Level Resolution | | 5 | 199 | Tue Sep 08 2026 |
| pre-trained UNITE ITS classifiers for QIIME 2026.7 and scikit-learn=1.7.1 | | 15 | 9394 | Mon Aug 31 2026 |
| Train a taxonomy classifier by extracting an amplicon region from a curated sequence alignment (using SILVA as an example). | | 0 | 113 | Wed Aug 12 2026 |
| RESCRIPt-evaluate | | 0 | 100 | Mon Jul 27 2026 |
| Naive Bayes classifier trained on a custom NCBI RefSeq V3-V4 database classifies almost all ASVs as same species | | 1 | 80 | Wed Jul 15 2026 |
| ITS Classifier: Extract reference reads - HiSeq: | | 2 | 89 | Sun May 31 2026 |
| ITS2 classification: identical sequences but different results (DADA2 vs merged) | | 6 | 146 | Tue Apr 07 2026 |
| MIMt database for 16S, 18S, ITS, 23S and 28S new release available (March 26) | | 0 | 136 | Wed Mar 18 2026 |
| Annotation and Classifier question | | 17 | 237 | Wed Mar 18 2026 |
| process killed qiime 2024.5 on Ubuntu 24.04.4 LTS when running on multicore | | 1 | 71 | Wed Mar 11 2026 |
| Integrating V3-V4 and V4 16S datasets for large-scale meta-analysis: Is Greengenes2 the optimal choice for cross-region alignment? | | 1 | 187 | Fri Mar 06 2026 |
| QIIME2-amplicon-2025.7 16S PacBio classification issues/discussion | | 6 | 182 | Tue Feb 24 2026 |
| The differences in taxonomy output by the feature-classifier trainer and the full-length trainer | | 6 | 150 | Sun Jan 18 2026 |
| "over-classification" of ASV taxonomy? | | 2 | 106 | Fri Jan 09 2026 |
| Troubleshoot classification where all ASVs are unassigned? | | 7 | 166 | Tue Dec 02 2025 |
| Evaluate custom taxonomic classifier with RESCRIPt? | | 5 | 203 | Thu Nov 27 2025 |
| How to perform SILVA V3 classifier training? | | 9 | 309 | Thu Dec 04 2025 |
| MIMt database new release!!! QIIME2 classifiers for markers 16S, ITS, 18S, 23S and 28S | | 0 | 142 | Mon Nov 03 2025 |
| Feature-clasifier V3-V4 | | 6 | 192 | Wed Oct 22 2025 |
| Trained classifier output not found | | 6 | 135 | Thu Sep 11 2025 |
| Question about consensus taxonomy assignment in classify-consensus-vsearch | | 6 | 229 | Thu Aug 21 2025 |
| Is there a pre-trained QIIME 2 classifier with full-length 16S (.qza)? | | 10 | 341 | Thu Aug 14 2025 |
| 16S SRNA | | 1 | 108 | Sat Aug 02 2025 |
| Greengenes2 and RESCRIPt | | 2 | 167 | Wed Jul 09 2025 |
| SILVA 138.1 classifier trained on version 2023.5 and used in 2024.5 | | 2 | 272 | Thu Jul 03 2025 |
| Dealing with Poor Taxonomic Assignments | | 6 | 212 | Tue Jun 24 2025 |
| Request for Pre-trained SILVA V1–V3 Naive Bayes Classifier | | 2 | 109 | Wed Jun 18 2025 |
| Using RESCRIPt's 'extract-seq-segments' to extract reference sequences without PCR primer pairs. | | 5 | 6796 | Mon Aug 17 2026 |
| Processing, filtering, and evaluating the SILVA database (and other reference sequence data) with RESCRIPt | | 8 | 66664 | Mon Jun 23 2025 |
