| Denoising low quality reverse reads in paired-end sequencing reads | | 4 | 25 | Wed Dec 03 2025 |
| Issue with UNITE classifier for ITS1 | | 1 | 14 | Tue Dec 02 2025 |
| Error was encountered while running DADA2 in R (return code 1) | | 8 | 44 | Mon Dec 01 2025 |
| DADA2 denoising taking long time | | 6 | 28 | Mon Dec 01 2025 |
| DADA2 Fails on AVITI Data | | 8 | 81 | Wed Oct 29 2025 |
| Questions about performing quality control with dada2 and generating a feature table | | 2 | 20 | Sat Nov 29 2025 |
| qiime dada2 denoise-paired is unresponsive | | 3 | 42 | Mon Oct 27 2025 |
| DADA2 priors trimming | | 1 | 11 | Tue Nov 18 2025 |
| priors in qiime2-dada2 | | 4 | 244 | Tue Nov 18 2025 |
| Observed ASV richness drops after adding 3rd MiSeq library — why do I lose ~100 species when I merge libraries? | | 4 | 23 | Thu Oct 16 2025 |
| ITS denoising truncation | | 2 | 30 | Wed Oct 15 2025 |
| Need help choosing truncation lengths for DADA2 and interpreting the demultiplexed sequence count summary. | | 2 | 37 | Mon Oct 13 2025 |
| QIIME2 non-chimeric reads | | 3 | 44 | Mon Nov 10 2025 |
| DADA2 -- denoise | | 2 | 52 | Fri Nov 07 2025 |
| Significant 16S sequence loss while merging w/ DADA2, various truncation settings used | | 2 | 80 | Wed Nov 05 2025 |
| Interpreting KEGG vs MetaCyc pathways in 16S (PICRUSt2) functional prediction | | 1 | 42 | Sun Oct 19 2025 |
| Trained classifier output not found | | 7 | 83 | Thu Sep 11 2025 |
| Converting 16S V3–V4 ASV reads to absolute copy numbers (qPCR / spike-in calibration?) | | 3 | 76 | Thu Oct 09 2025 |
| Chimera removal dilemma in 16S V3–V4: necessary when using Kraken2/Bracken? | | 8 | 93 | Fri Sep 26 2025 |
| 16S CCS without primers: how to skip --p-front/--p-adapter in denoise-ccs? | | 4 | 66 | Tue Aug 26 2025 |
| Effect of primer removal on chimera detection in DADA2 | | 11 | 163 | Mon Aug 25 2025 |
| Why is non-chimera read count often referred to as sequencing depth in 16S rRNA analysis? | | 4 | 100 | Thu Sep 18 2025 |
| Percentage of reads discarded after running DADA2 on NextSeq data | | 1 | 49 | Mon Aug 25 2025 |
| Less percentage of reads merged in qiime-DADA2 even with enough overlapping length present. | | 7 | 78 | Thu Jul 10 2025 |
| dada2 error: rcpp/SparseArray | | 2 | 33 | Wed Jul 09 2025 |
| Shall I need to remove ASV with prevelance in one sample? | | 4 | 63 | Fri Aug 08 2025 |
| Why is there a size difference between my PCR product (~400 bp) and the final representative sequence (313 bp)? | | 4 | 85 | Tue Jul 22 2025 |
| Please help interpreting demux data | | 2 | 66 | Tue Jun 17 2025 |
| Minimum length of read in ASV (DADA2) | | 6 | 113 | Sun Jun 08 2025 |
| PICRUST2 Input Data setting | | 1 | 58 | Tue Jul 08 2025 |
